Effect of Seminal Plasma on Hipoosmotic Swelling Test in Fresh Alpaca Spermatozoa

The process of fertilization can’t be attributed only to the number of live, motile and normal sperm deposited within the female but especially to its functionality [1]. The hypoosmotic test or hypoosmotic test (HOST) is a functional test, by which the live sperm are subjected to an incubation in a hypoosmotic solution, the sperm with the functional membrane will allow the entry of water by osmosis that will be evidenced by swelling and curling of the tail [2]. In ejaculates of llamas obtained by artificial vagina, and subjected to said test, endosmosis percentages of 40% were observed using a solution of 100 mOsmol [3]. Other reports in flames indicate 33.48% in semen collected by electro ejaculation and 30.15% in semen collected by artificial vagina using hypoosmotic solution adjusted to 50 mOsmol [4]; and a last report indicates 36% in semen sperm collected by burning electro ejaculation using also hypoosmotic solution adjusted to 50 mOsmol [5]. In alpaca ejaculates obtained by electro ejaculation, percentages of endosmosis were obtained with a range of 20 to 62% using 100 mosmol hypoosmotic solution [6], while in alpacas whole semen 23.5% was reported using hypoosmotic solution of 150 mOsmol [7]. Alpaca epididymal spermatozoa were subjected to the hypoosmotic test at an osmolarity of 100 mOsmol, obtaining a response of 89.08%, evidencing a greater response in free sperm from seminal plasma [8]. The artificial vagina method allows obtaining complete ejaculates of alpacas [9], free seminal plasma sperm can be obtained by means of the deferential vas deferens technique permanently [10]. Under these considerations, the objective of the present investigation was to evaluate the endosmotic response of spermatozoa of alpaca in the presence and absence of seminal plasma and reconstituting the seminal plasma to sperm obtained from the vas deferens.